LOCUS       40924_49122        3850 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD022, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3850)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3850)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..536
                     /note="S7942NS2-RA; right arm of the homologous
                     recombination site NS2 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U44761"
     misc_feature    445..450
                     /label=SacI
                     /note="SacI"
     rep_origin      573..1161
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            complement(1402..1511)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_recomb     1698..2639
                     /note="S7942NS2-LA; left arm of the homologous
                     recombination site NS2 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U44761"
     misc_feature    2640..2645
                     /label=NheI
                     /note="NheI"
     misc_feature    2646..2666
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    2664..2669
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(2793..3650)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3651..3755)
                     /label=AmpR promoter
     misc_feature    3781..3786
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagcgacgt cgggggcccc
       61 cggggggatt ctagagttgc taccaagtag tgacccgctt cgtgatgcaa aatccgctga
      121 cgatattcgg gcgatcgctg ctgaatgcca tcgagcagta acgtggcacc ccgcccctgc
      181 caagtcaccg catccagact gaacagcacc aagaggctaa aacccaatcc cgccggtagc
      241 agcggagaac tacccagcat tggtcccacc aaagctaatg ccgtcgtggt aaaaatcgcg
      301 atcgccgtca gactcaagcc cagttcgctc atgcttcctc atctaggtca cagtcttcgg
      361 cgatcgcatc gatctgatgc tgcagcaagc gttttccata ccggcgatcg cgccgtcgcc
      421 ctttcgctgc cgtggcccgc ttacgagctc gtttatcgac cacgatcgca tccaaatccg
      481 cgatcgcttc ccagtccggc aattcagtct ggggcgtccg tttcattaat cctgatagcg
      541 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      601 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      661 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
      721 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      781 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      901 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      961 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1021 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1081 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1141 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1201 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     1261 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     1321 tcagtgagcg aggaagcgga agactggttg gcttggtttc atcagccatc cgcttgccct
     1381 catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg
     1441 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac
     1501 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacgcga accctttggc
     1561 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc
     1621 cgaagcaggg ttatgcagcg gaagatccct gacgggcttg tctgctcccg gcatccgctt
     1681 acagacaagc tccgcgggcc acgatttgag ggacgaatct tcagcgtgac tgaagtacct
     1741 cgcggcaagc catttcccga tgtctttttg ttggccgccg atcgcttcgg ggttaatcct
     1801 acggcctgcg ctgtgatcga agacaccccc ttgggagtag cggcaggcgt ggcggcagga
     1861 atgcaagtgt ttggctacgc gggttccatg cccgcttggc gtctgcaaga agccggtgcc
     1921 catctcattt ttgacgatat gcgactgctg cccagtctgc tccaatcgtc gccaaaagat
     1981 aactccacag cattgcccaa tccctaaccc ctgctcgcgc cgcaactaca cactaaaccg
     2041 ttcctgcgcg atcgctctta ctgttgatgg ctcgtgctta aaaacaatgc aaccctaacc
     2101 gtttcagctg gtgattttcg gacgatttgg cttacaggga taactgagag tcaacagcct
     2161 ctgtccgtca ttgcacaccc atccatgcac tggggacttg actcatgctg aatcacattt
     2221 cccttgtcca ttgggcgaga ggggagggga atcttctgga ctcttcacta agcggcgatc
     2281 gcaggttctt ctacccaagc agtggcgatc gcttgattgc agtcttcaat gctggcctct
     2341 gcagccatcg ccgccaccaa agcatcgtag gcgggacgtt gttgctccag taaagtcttc
     2401 gcccgtaaca atccccagcg actgcgtaaa tccgcttcgg caggattgcg atcgagttgc
     2461 cgccacagtt gtttccactg ggcgcgatcg tcagctcccc cttccacgtt gccgtagacc
     2521 agttgctctg ccgctgcacc ggccatcaac acctgacacc actgttccag cgatcgctga
     2581 ctgagttgcc cctgtgcggc ttcggcttct agcgcagctg cttggaactg cacacccccg
     2641 ctagcatcgc gcgcgcgcgc gcggacgtcc tcacgttaag ggattttggt catgagatta
     2701 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2761 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2821 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2881 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2941 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3001 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3061 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3121 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3181 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3241 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3301 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3361 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3421 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3481 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3541 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3601 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3661 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3721 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3781 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     3841 ccctttcgtc
//
